Art Essay Example | Topics and Well Written Essays - 1250 words

Workmanship - Essay Example In Cassatt’s work, she utilizes light hues and maintaining a strategic distance from dark creating an exceptionally sentimental canvas that delineates a mother and youngster. In Manet’s works, he dismisses what was the decree of impressionism by his trademark utilization of dark. The two specialists are working from a Japanese style of structure and point of view which as per numerous reports intensely impacted their canvases. As per pbs.org, Manet’s paints his structures rapidly in unpleasant brushstrokes unmistakably noticeable on the outside of the canvas. Rather than the painstakingly developed point of view that drives the eye profound into the space of the artistic creation, Manet offers an image outline leveled into two planes. The frontal area is the shining white collection of Olympia on the bed; the foundation is obscurity. (Edouard Manet’s Olympia). The treatment of the topic is totally different in the two works of art. As per pbs.org, Manet de cides to paint a lady of his time - not a female perfect, yet a genuine lady, and a mistress at that.(Edouard Manet’s Olympia).This lady is a confident whore and perspective on the general population of the nineteenth century an outrageous canvas which would have been crushed by the general population as announced by numerous individuals. As indicated by the Guardian, â€Å"The topic is hair-raising - a bare who, according to nineteenth century spectators, was obviously a whore, in her trashy donkeys with a bootlace for a neckband, took care of, to the unsettled white cushions and her skin is a brilliant, glaring white. The completely dressed dark worker is included, giving her blossoms from one of her male companions.†(Olympia, Edouard Manet).In seeing the composition the whore unmistakably has a resistant and angry look which as per the Guardian, it is difficult to know whether her mind-set is one of despairing or disdain.( Olympia Edouard Manet(1863)) On the other h and, Cassatt’s mother and youngster are considerably more simpler and pleasurable to see. The â€Å"mother and child† plainly delineate the close love and bond between a mother and her youngster. As per Danielle Peltakian, â€Å"she painted in fragile brush strokes of pastel shades average of the style of Renoir and Degas. She made a sentimental delineation of the female figure suggestive of the early Italian renaissance Madonna and child†( American individual from the french impressionists school:note print producer.) According to tfaoi.com, The mother in the canvas radiates a feeling of respect and reason that challenges ordinary ideas of enlivening femininity.(Mary Cassat, Modern woman).According to Anne Higonet, Cassatt made the experience of minuscule kids' bodies an instinctive joy, both for the moms spoke to inside her photos and for us, the watchers of the pictures(Two perspectives about Cassatt).She goes further in clarifying that Cassatt's image joins the types of mother and kid. The trustworthiness of the individual body, which we could likewise call its disengagement, is supplanted by a proper fiction of combined bodies. Cassatt draws in us in the delights of baby tissue. (Two different ways of pondering Cassatt). In looking at the two pictures by Cassatt and Manet, the distinctions in delineation of ladies is monstrous. In manet’s Olympia, the lady is portrayed as an item, while in Cassatt’s Mother and youngster the lady is a subject. Mary Cassatt portrayed the advanced lady of the Paris. A women's activist point of view of her work plagues the greater part of the clarification and investigation of her work which portray her as communicating her

The Effects of Equality Act 2010 on NHS Employment Dissertation

The Effects of Equality Act 2010 on NHS Employment - Dissertation Example Equity act 2010 all the open help associations working in the UK legitimately or by implication. The organization's need to reframe their client support norms, change the strategy for grievance taking care of and give more consideration to representative related issues. Each organization needs to help a report guaranteeing there is no sex based, race-based or incapacity based segregation in their office demonstrating the equivalent through factual information (Briefing 74, 2010).NHS or National Health Service is an exceptionally celebrated trust giving wellbeing administrations to specific individuals in the UK. The trust handles about 9 million calls every year and over 1.5 million site arranged solicitations. More than 3000 staff works with NHS picking up the calls, booking arrangements and going to crisis cases (NHS Direct, 2011). Individuals from various sort of classes, races and money related foundation call NHS every day. NHS is the best association to screen how the uniformit y demonstration 2010 influences the business methodologies of an association straightforwardly and in a roundabout way. Before staying profound into the exploration technique let us break down the historical backdrop of the Equality demonstration 2010 in detail.The Equality act 2010 was encircled after exact assessment of different political and social conditions. The group of specialists investigated the authentic premise on which the past laws were framed and disposed of more than 100 little classes and subsections to develop them all under one single act. A few remarkable bits of antidiscrimination laws like Equal Pay Act 1970 The Sex Discrimination.

Random Hall Rooms

Random Hall Rooms Random Hall has six types of rooms, discernible by the last digit of their room numbers. Each of the four floors is concentric with the shaft, a rectangular hole through the center of the building. The dorm consists of two adjacent chiral buildings, 282 Massachusetts Avenue and 290 Massachusetts Avenue, joined at the basement, the first floor, the third floor, and the roofdeck. Each building has its own four floors and its own shaft. Each floor, with the exception of the two first floors, looks like this: 5-room, large half: My home for the past three semesters has been 335, the wonderful lounge-side 5-room on the 290-side third floor. My door opens up to the hallway connecting my floor’s lounge to the back of the building, making it visible from the third-floor portal to the 282 side of the building. If my door is open I have a strategic view of the most popular intersection in the dorm. If my door is closed my room is as quiet as the rest of the back of the building. 335 was initially painted a beautiful light purple that glowed orange in the sun. Unfortunately the paint was old and cracked and had handprints suspiciously close to the ceiling. Before my sophomore year I finally painted it the three shades of green you can see below, supplied by Random Hall for free: Below are some of my favorite things in my room. On the left wall above (click for a larger view) and on the upper left below is the painting my amazing then-eight-year-old brother gave me when I left for MIT. On the wall above my bed is possibly my favorite photo ever, taken in Israel by a family friend. The glass of the frame broke on the flight home. The fake wooden board behind it was the back of a shelving unit I brought with me to MIT but never actually attached, despite everyone’s insistence that I had doomed the shelving unit to collapse. Probably as a result, the top half of the shelving unit did collapse partway through my first semester. The bottom half survived in my closet until I got annoyed at the exposed screws; I’m not sure if I got rid of it then or if it finally buckled. When I moved into my current room I nailed the cardboard to the wall. (Id like to pretend it was symbollic but it wasnt; the wall just felt empty.) Hippie the hippo was given to me by my mom’s boss’s wife when we first moved to Chicago 16 years ago. The giant turtle was from the same era, and the stuffed cat was a Christmas present that cost around $20, which at the time was a lot of money. The lion and the beaver are more recent: I got the lion overpriced at a zoo giftshop because I really, really wanted him, and the beaver was my personal prize for getting into MIT. /div I also have an exceptional fish, a delta tail male betta fish I bought this summer and named Hephaestus, after the Greek god of the blacksmiths fire, in hope that his ceaseless swimming and red fins would inspire my own energy and productivity. He has not in fact magically inflated my efficiency, but it has been reassuring to come home every evening to him still swimming, no matter what’s going on outside his fishbowl. I made you an ominous video of Hephaestus eating, set to Clint Mansell’s “Lux Aeterna,” the most ominous music I could find: amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;br /amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp ;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 5-room, small “half”: If you look back at my current room you might notice a dark green doorway just visible above the shelves on the right wall. This door leads to 335s, the room I lived in during my first semester (the same door is on the left in the photo below): 335s is 335’s small half, though it is only a half to the extent that one third is a half. The small half is about half the size of the large half, just wide enough to fit a bed. Living in a small half is a good lesson in keeping your floor as clean as possible and taking advantage of vertical space. It can be a pleasant, cozy room if organized well. Like the large half it faces the alleyway, which means it is relatively quiet and gets a lot of light. Unlike the large half it has a closet, which is ironic. Small halves are usually freshman rooms. Though they are small, they are practically singles: they are considered doubles only because of the door and the ease of listening in on the large half’s occupant. An even better perk is that the resident of the small half inherits the large half as soon as it is empty. You can barely see it in this photo, but this room is painted three shades of yellow. My close friend Dana ‘14 painted it to match my green stripes when she lived in it last fall. It is currently vacant, which means that one of you will probably live in it next term. 2-room: The 2-room is the only real double in Random Hall and the other usually freshman room. 2-rooms have doors facing lounges or kitchens and windows facing Massachusetts Avenue, which makes them noisier than most other rooms but also makes it much easier to socialize, since you dont even have to leave your room to see people. This particular room is 342, which opens up into the kitchen of my floor. 1-room: 1-rooms are singles adjacent to 2-rooms. Like 2-rooms, 1-rooms have doors facing lounges or kitchens and windows facing Massachusetts Avenue, making them noisy and social. 1-rooms have one window, which is larger than other rooms’ windows. This is 221, a lounge-facing 2-room on the second floor with what I think is the coolest paint job in the dorm. It belonged to Rebecca ‘12 last year and is now Ellen ‘14’s. 3-room: 3-rooms have two windows looking out into the shaft. Lounge-side 3-rooms have large walk-in closets, which some of my friends have used as separate study rooms, while kitchen-side 3-rooms do not. This is 423, a lounge-side 3-room that belongs to Mika ‘14 during term and is Ceres ‘14’s for the summer. 4-room: 4-rooms are divided into two halves separated by arches. The first half has a door to the hallway. The second half has a window looking out into the shaft. This is Cory ‘13’s room. I painted the walls grey and navy blue over the past month. They were originally blank Institute white, which can drive a person crazy. Above is the first half of the room, viewed from the door. Below is the second half, with the arches on the left. My favorite part of Cory’s room is without a doubt his plushies. The story goes that Mustache Bunny Theodore, wanting a big, strong son, mated with a cow, a tiger, and a zebra to produce Montgomery, Winston, and Claudius, respectively, pictured below. After three attempts produced striped or spotted mustached rabbits of normal rabbit size, Mustache Bunny adopted Buffalo, who will presumably grow up to be buffalo sized. Here they are shown as I found them when I walked in last Thursday. I don’t know what plots they concoct when we’re not there, but they must be important. Hopefully this has been helpful to those of you who might want to live here. Please comment if you have questions about rooms or housing, or if you find out in two weeks that youve been placed in Random Hall. =) Post Tagged #Random Hall

The Conflict Between Antigone And Creon - 836 Words

In Sophocles’ tragedy, Antigone, Sophocles follows Aristotle’s definition of tragedy which is constructed in six parts: plot, character, diction, thought, spectacle, and song. Although these six parts are used to produce a successful tragedy, thought is especially important in Antigone because it encapsulates the plot of the play. The thought, or the theme, is revealed in the dialogue from the Prologue to the Second Episode. As a result, Antigone’s and Creon’s dialogue exhibits two interrelated themes: personal convictions versus duty to the state and human law versus divine law. Although both themes develop the conflict between Antigone and Creon and portray the personality differences between the two, both of their beliefs cause their demise thus proving there is no right form of justice. The theme, personal convictions versus duty to the state, depicts the contrasting values between Antigone and Creon. In the Prologue, Antigone discusses with her siste r, Ismene, her plans to bury Polyneices, their brother, even though he has been condemned an enemy of the state by Creon for his disloyalty to Thebes. After Ismene refuses to join her and attempts to talk about the consequences, Antigone states that â€Å"he is my brother still†¦ but I shall not abandon him† (345). This shows Antigone’s loyalty to her family despite what Creon dictates. The Prologue not only introduces the relationship between the sisters but also their contrasting values and personalities. Ismene prefers toShow MoreRelatedThe Conflict Between Antigone And Creon1353 Words   |  6 PagesSophocles’ play Antigone told the story of a young woman, Antigone, who was willing to violate the law for her family. In the play, Antigone’s brothers, Eteocles and Polynices, killed each other in battle. Creon, King of Thebes, buried Eteocles’ body because he fought in defense of Thebes; however, Creon issued a decree forbidding the burial o r mourning of Polynices, because he had fought against Thebes. While this play’s central plot told the story of a conflict between Antigone and Creon, due to hisRead MoreThe Conflict Between Antigone And Creon1327 Words   |  6 PagesAntigone is a play that was written in ancient Greece by the playwright Sophocles. It is the third play in a trilogy of tragedies about the city-state of Thebes, revolving around Oedipus Rex. Antigone starts the day after a civil war fought between the two sons of Oedipus Rex after his death. The civil war ended in death for both brothers, so their uncle, Creon, assumed the role of King of Thebes. The main conflict of the play begins when Creon gives one brother, Eteocles, a burial with honors, butRead MoreThe Major Conflict Is Between Creon And Hero Antigone1074 Words   |  5 Pages The major conflict is between Creon and hero Antigone. The conflict was over the decision to determine which principles were most valuable. This story d epicts the conflict of whether the law of the gods or the law of the city is the proper way to go. However, one would argue that we have the power to make choices that will affect not only our lives, but the lives of other people. In our everyday life we’re always faced with situations like Antigone that causes us to make wise sound choicesRead More Conformity and Rebellion in Antigone Essay769 Words   |  4 PagesWould you rather not have conflicts in your life? Antigone is a play, based upon conflicts between the characters. Antigone is the sister of Polynices, who is dead. Polynices is considered a traitor to Creon and he doesn’t bury those who are against him. As conflicts occur, Antigone, Creon, and the Chorus argue on whether to have Polynices buried or not. Conflicts are a necessary part of life because of the changes that we run in to. It is appropriate to rebel and create conflict when we believe in doingRead MoreEssay on Moral Conflict in Antigone1349 Wor ds   |  6 PagesThe major moral conflict in Antigone by Sophocles is the conflict over which value is most fundamental. The play presents the moral conflict over whether the gods law or the citys law is more powerful. This seems to be the most prominent theme. The conflict arises mainly between the tragic heroes Antigone and her uncle-in-law Creon, King of Thebes. The city of Thebes had been through a war in which Antigone and her sister Ismene have lost both of their brothers to it, Eteocles and PolyneicesRead MoreThe Punishment For Overstepping Creon s Authority Essay1235 Words   |  5 Pagesmore sinister. In Sophocles’ â€Å"Antigone† there is a clear gender conflict that forms between the protagonist Antigone and the antagonist Creon. Antigone’s determination to give her brother a just burial inspired the people of Thebes, but enraged Creon. Although Antigone does not obsess over the gender struggle as much as Creon, there is no disputing that the g ender roles of Ancient Greece fueled the central conflict of the drama. The clash between Antigone and Creon was symbolic towards the subjugationRead MoreBiography of Sophocles Essay1496 Words   |  6 Pageslifetime, Sophocles composed more than one hundred plays, of which seven complete tragedies and fragments of eighty or ninety others are preserved. Of the complete works available, Antigone, Oedipus Tryannus (Oedipus Rex), and Oedipus at Colonus are widely considered to be masterpieces (Terrell 2). 2. Summary: Antigone is a play which embodies many values held dear to the ancient Greeks: morality, pride, selfless love, the power of the Gods, and similar issues which are timelessly inherent withinRead MoreConflict In Sophocless Antigone1507 Words   |  7 Pagesto the conflict in Antigone since my friends and I thought our decisions were the right things to do at the moment and did not think of the consequences. In the play Antigone, written by Sophocles, Antigone, Creon, and the gods were all to blame for all the issues that occurred in Thebes. The writing, Antigone, is about the struggle between knowing what is right and wrong when two laws contradict one another. The story takes place in a city-state, Thebes, in Ancient Greece. The main conflict in theRead MoreThe Conflict Of Sophocles Antigone1500 Words   |  6 PagesSophocles’ Antigone centers around a familial feud that develops between Antigone and Creon when Antigone decides to bury her brother and Creon’s niece, P olyneices. While Antigone believes that it is her religious and familial duty to bury her brother, Creon objects, citing the Theban civil war which took place right before the events of the play. Adhering to Greek literary tradition, Sophocles ultimately seeks not just to entertain the audience but also to teach a moral lesson, in this case aboutRead MoreAntigone : Human Law Vs. Divine Law1418 Words   |  6 PagesLauren Wolfson Professor Neil Scharnick THR 3270 History of Classical Theatre 30 November 2016 Antigone: Human Law vs. Divine Law The most prominent theme in â€Å"Antigone† by Sophocles is the conflict of divine law vs. human law; it is the driving force behind the entire play. It is an issue of which law is the right law, and if Creon s and Antigone s acts were justifiable. It is also a question of what motivates them both to act as they do and if they were predestined to their fates due

Okinawa Geography and 10 Fast Facts

Okinawa, Japan is a prefecture (similar to a state in the United States) that is made up of hundreds of islands in southern Japan. The islands comprise a total of 877 square miles (2,271 square kilometers) and has a population of over 1.3 million. Okinawa Island is the largest of these islands and its where Naha, capital of Okinawa Prefecture, is located. Okinawa made headlines around the world when a magnitude 7.0 earthquake struck the prefecture on February 26, 2010. Little damage was reported from the earthquake, but a tsunami warning was issued for the Okinawa Islands, as well as the nearby Amami Islands and the Tokara Islands. There are ten important facts to know when learning about or  traveling to Okinawa, Japan: The main set of islands making up Okinawa is called the Ryukyu Islands. The islands are then further divided into three regions called the Okinawa Islands, the Miyako Islands, and the Yaeyama Islands.Most of Okinawas islands are made up of coral rocks and limestone. Over time, the limestone has eroded in many places throughout the various islands and as a result, many caves have formed. The most famous of these caves is called Gyokusendo.Because Okinawa has abundant coral reefs, its islands also have a plethora of sea animals. Sea turtles are common in the southernmost islands, while jellyfish, sharks, sea snakes, and several types of venomous fish are widespread.Okinawas climate is considered subtropical with an average August high temperature of 87 degrees F (30.5 degrees C). Much of the year can also be rainy and humid. The average low temperature for January, Okinawas coldest month, is 56 degrees F (13 degrees C).Because of its climate, Okinawa produces sugar cane, pineapple, pap aya, and has many botanical gardens.Historically, Okinawa was a separate kingdom from Japan and was controlled by the Chinese Qing Dynasty after the area was annexed in 1868. At that time, the islands were called Ryukyu in native Japanese and Liuqiu by the Chinese. In 1872, Ryukyu was annexed by Japan and in 1879, it was renamed the Okinawa Prefecture.During World War II, there was a Battle of Okinawa in 1945, which led to Okinawa being controlled by the United States. In 1972, the United States returned control to Japan with the Treaty of Mutual Cooperation and Security. Despite giving the islands back to Japan, the U.S. still maintains a large military presence in Okinawa.Today, the United States currently has 14 military bases on the Okinawa Islands, most of which are on Okinawas largest main island.Because Okinawa was a separate nation from Japan for much of its history, its people speak various languages that differ from traditional Japanese.Okinawa is known for its unique arch itecture that developed as a result of frequent tropical storms and typhoons in the region. Most of Okinawas buildings are made of concrete, cement roof tiles and covered windows. Sources Mishima, Shizuko. The Okinawa Islands, Mapped Out. Trip Savvy, March 26, 2019.

Lord Byron Free Essays

In `Excerpt from Don Juan`, answer the following: Select 3 stanzas from Canto I that you can explain. Do not give plot summary, so carefully choose a stanza that lends itself to analysis or some research. Write about three to four sentences. We will write a custom essay sample on Lord Byron or any similar topic only for you Order Now In Subject Line, identify your stanza, e.g., 44 (Canto I, Stanza 44). Stanza 5 Brave men were living before Agamemnon And since, exceeding various and Sage, A good deal like him too, though quite the same none;  Ã‚   35 But then they shone not on the Poet’s page, And so have been forgotten: – I condemn none, But can’t find any in present age Fit for my poem (that is, for my New One) So, as I said, I’ll take my friend Don Juan. –  Ã‚  Ã‚   40 In analyzing Stanza 5, it’s interesting to read as we wonder who else Byron may have considered in this poem he sat down to write. Was he considering other brave men, poets, heroes before finalizing it with Don Juan? Who were the others â€Å"a great deal like him† (Canto 1, Stanza 5, Line 35). This stanza can be more interesting to readers who examine the history of what was viewed as the scandalous controversial nature of his writing of â€Å"Don Juan† and what concessions did Byron have to end up making before it was published. Stanza 5 sums up what he was saying about heroes in the previous stanzas. Stanza 6 Most epic poets plunge â€Å"in medias res† (Horace makes this the heroic turnpike road), And then your hero tells, whene’er you please, What went before—by the way of episode, While seated after dinner at his ease,  Ã‚   45 Beside his mistress in some soft abode, Palace, or garden, paradise, or cavern, Which serves the happy couple for a tavern. The analysis of Stanza 6 lets the reader in that Byron is intentionally not following Horace’s recommendation of when to start an epic. Byron is (intentionally?) not following the rules of what at the time was being seen by other writers as the better way of starting an epic, which was in the middle. This stanza proves to us the writer is choosing not to write using the examples of Homer or Virgil but writing this epic his own way (Canto 1, Stanza 6, Lines 41-44). Stanza 7 That is the usual method, but not mine— My way is to begin with the beginning;  Ã‚   50 The regularity of my design Forbids all wandering as the worst of sinning, And therefore I shall open with a line (Although it cost me half an hour in spinning) Narrating somewhat of Don Juan’s father,  Ã‚   55 And also of his mother, if you’d rather. Although the reader isn’t aware of it until later, Byron reveals to us that he knew in advance he would digress in the poem (Canto 1, Stanza 7, Line 54). The reader now learns that was the writer Byron’s intention from the start. The reader can wonder if Byron is even conscious of how he changes some of the â€Å"traditional† epic writing in writing this work. In `Excerpt from Childe Harold`s Pilgrimage`, answer the following: 1. Does the Byronic hero know any form of Keatsian love? Yes, in that Keatsian love is often associated with â€Å"beauty-as-truth.† In Canto 2, Stanza 9 we read as Byron writes of having loved and it was still in his thoughts although he is now alone with those thoughts. We also read of this â€Å"beauty-as-truth† love in Canto III Stanza I when he relates of the love for his daughter. 2. Beginning with stanza 17, the narrator talks about Waterloo. Why? Waterloo is current to at this time to Byron. Just a few months before this, the fate of Europe had been decided because of that Battle. So it is important that the reader is aware that it is sacred ground to him. The battle was fought on June 18th, 1815 which makes this a very relevant event during his lifetime of 1788-1824. 3. In what ways is this poem about mid-life crises? Childe in this epic refers to a â€Å"knight† and we read as this knight is gloomily wandering as a vicious world-worn man. In his thoughts throughout the â€Å"pilgramage† it relates closely to a man who is going through similar thoughts a man in mid-life crises might go through as though he has already fully lived. 4. How does the Byronic hero relate to nature? Byron relates better to nature than he does to humans. In Canto 4, Stanza 178, Byron states this: There is a pleasure in the pathless woods, There is a rapture on the lonely shore. There is society where none intrudes, By the deep Sea, and music in its roar: I love not Man the less, but Nature more, From these our interviews, in which I steal From all I may be, or have been before, To mingle with the Universe, and feel What I can ne’er express, yet cannot all conceal However, in reading this piece, I feel its obvious throughout to the reader that the writer can connect more easily with nature than humans. Because most of Byron’s work is autographical in nature, this is easy to understand if the reader about Byron’personal life. References â€Å"Characteristics of the Byronic Hero.† University of Michigan. Online. Internet. 17 May 2003. (2002, February 11). Childe Harold’s Pilgrimage. Retrieved May 17, 2007, from The Project Gutenberg Web site: http://www.gutenberg.org/dirs/etext04/chp110h.htm (2007). George Gordon, Lord Byron. Retrieved May 17, 2007, from Bob’s Byway Web site: http://www.poeticbyway.com/xbyron.html ; ; How to cite Lord Byron, Essay examples

Core Curriculum for Critical Care Nursing

Question: Discuss about the Core Curriculum for Critical Care Nursing. Answer: Introduction: The paper is an outcome of personal reflection as a part of learning and development in nursing practice. The reflection is based on evaluation of personal thoughts, own practice, feelings, experiences and actions. The model of reflection which I most commonly prefer are Gibbs Reflective cycle (1998) and Rolfes framework (Saunders et al. 2016). As a registered nurse my experience in the area of critical care so far have been exciting and interesting. Currently, I am highly competent in handling medical emergencies, preparing the patients for the procedures, assisting physicians, and administering treatment. I have always received appreciation from my peers and team leader for my good verbal communication skills and ability to make quick and sound decision for acute and critical care patients. These skills are the area of strength that makes me confident to handle difficulties. I remember an incident where I walked into the critical care unit and realised it is going to be a busy day. I have collected reports of Mrs. Luigi, 65 whom I was suppose to care that day. She was under incubation and was sedated after hip surgery. She was surrounded with equipments and tower of I.V. infusions pumps. I have finished quick assessment of her ensuing not to miss any details though I was busy. I found her stable. Next to it I was showered by lots of questions by her anxious family. I brought them to the bedside and considering them as my own patient I have answered all the questions and gave sufficient and relevant information related to Luigi. I reassured them while I continue to perform my assessment and care. To cater for their emotional and spiritual needs I have asked a chaplain to visit the family members. My assessment was crucial for the multidisciplinary team and the team was waiting for my feedback on Luigi. Later, I found that many of my suggestions were implemented and after 24 hours I could see her condition improving. While preparing reports I have felt a strong sense of achievement. However, my weakness includes lack of cross-cultural skills that is linguistic barrier when attending patients from CALD community. I am on way of improving this skills since last year. There are areas of improvement as per my mentors feedback and personal experiences with patients of different backgrounds. Although I am able to implement the evidence-based options and acknowledge positive decisions and actions of my senior nurses, I fail in certain areas. These include unable to communicate with the patients who are deaf and blind and unable to handle aggressive patients in ICU. There is a need of setting goals for improvement in these areas. According to Alspach (2013) having clear goals is vital for self-regulated learners. They are motivated towards accomplishing these goals. Revello and Fields (2015) describes that more the distance of an individual from his/her goals, the more is the investment of time and resources. Therefore, short term and long-term goals must be set to achieve the regular targets and progress towards the ultimate goal. As a registered nurse, I have identified a set of short-term goals in relation to areas of improvement identified which would be practical for me to achieve by the end of the end of Critical Care 2. In relation to the areas of improvement identified, I have set short-term goals which I aim to achieve by the end of critical care 2. These are- Short term goals Resources What to learn Learn non verbal skills particularly gesture and ESL Take assistance from senior nurse managers who are undertaking cases of deaf or blind patients in ICU and work with them for one month and work with interpreters (Chininthorn et al. 2016) how to perceive, comprehend and transmit messages when interactive with patient with sensory disability Learn ASL and gestures required for such patients Handle stress due to aggressive patients Attend workshops Help from colleagues Attend yoga and meditation classes after shift time daily for two months (Chang et al. 2016) how to report mentor when in emergency Develop mental resilience Act without taking personally Observation and build therapeutic connection with schizophrenic patients Handle abusive behaviour By the end of this course, I would like to demonstrate my improvement in the above mentioned in the area by up taking at least one case of mental health department for example schizophrenia. I would maintain portfolio of my personal improvements including evidence of my improvement. I would work with nurse manager for handling patients with visual impairment to improve my performance. I would like to develop transformational leadership skills as my mentor is expert in inspiring others and always have strong vision. She makes me feel passionate about success. Therefore, I am on way to cultivate this leadership trait to improve performance and reach managerial position in future as highlighted in Lin et al. (2015). References Alspach, J.G., 2013.Core curriculum for critical care nursing. Elsevier Health Sciences. Chang, S.J., Kwak, E.Y., Hahm, B.J., Seo, S.H., Lee, D.W. and Jang, S.J., 2016. Effects of a Meditation Program on Nurses Power and Quality of Life.Nursing science quarterly,29(3), pp.227-234. Chininthorn, P., Glaser, M., Tucker, W.D. and Diehl, J.C., 2016. Exploration of Deaf Peoples Health Information Sources and Techniques for Information Delivery in Cape Town: A Qualitative Study for the Design and Development of a Mobile Health App.JMIR Human Factors,3(2). Lin, P.Y., MacLennan, S., Hunt, N. and Cox, T., 2015. The influences of nursing transformational leadership style on the quality of nurses working lives in Taiwan: a cross-sectional quantitative study.BMC nursing,14(1), p.33. Revello, K. and Fields, W., 2015. An educational intervention to increase nurse adherence in eliciting patient daily goals.Rehabilitation Nursing,40(5), pp.320-326. Saunders, R., Singer, R., Dugmore, H., Seaman, K. and Lake, F., 2016. Nursing students reflections on an interprofessional placement in ambulatory care.Reflective Practice,17(4), pp.393-402.

Assess the challenge of Post-development theory to mainstream development paradigms Essay Example

Assess the challenge of Post Assess the challenge of Post-development theory to mainstream development paradigms Essay Assess the challenge of Post-development theory to mainstream development paradigms Essay Essay Topic: All Quiet On the Western Front The rise of Post-development theory in the late 1980s through to the 1990s advocated by scholars across the globe (Sachs, Escobar, Esteva, Shiva and Illich to name but a few) brought to the fore more radical interpretations and critiques of mainstream development paradigms. The post-development theorists set about a brutal yet arguably necessary attack upon current development practices and theories claiming to uncover some of the hidden truths behind the Western development project, as Esteva states The time has come to unveil the secret of development and see it in all its conceptual starkness (1992:7). Post-development embarked on a complete rejection of current development practice naming it a failure in every sense. However, others were sceptical, many believing that such a position was unnecessary and indeed unhelpful in terms of suggesting development alternatives, as Nederveen-Pieterse writes Post-development is caught in a rhetorical gridlock. Using discourse analysis as an ideological platform invites political impasse and quietism. In the end post-development offers no politics besides the self-organising capacity of the poor, which actually lets the development responsibility of the states and international institutions off the hook (2000: 187). Under such stark criticism the question often posed is what real challenge does post-development theory have to offer to the wider debate and reality of the development situation, if all it appears to be is semantic hot air? This paper will discuss in detail this very point, arguing that despite its at times, extreme radical view points, post-development has much to offer in terms of challenging our neoclassical interpretations and understanding of mainstream development theory. An initial overview will be given of the progression of development over the last four decades, highlighting the rise of post-development theory in the 1980s through to the 1990s. Following this, an in-depth assessment of the challenges posed to mainstream development by post-development will be given stressing the complexities associated with such challenges. Case studies and critique will be apparent throughout. Post-development theory grew out of a huge sense of dissatisfaction and disillusion with the way mainstream development theory was both constructed and operated. Such mainstream development has been seen as intrinsically linked to Neoliberal policies of economic reform and a dominant western understanding of how countries should progress and grow along the same teleological path as Western societies, the end goal of which being modernization and industrialisation. Mainstream development appeared to be constructed of a single, monolithic and imperialist vision of progress and planning as Escobar notes the idea that poor countries could move more or less smoothly along the path of progress through planning has always been held as an indubitable truth (1992:64). Post-development thinkers date the beginning of mainstream development to 1949 when President Truman made his famous speech, during which as Esteva believes, two billion people became burdened with the label underdeveloped (1992:7). Since that date development theory and practice has moved hap-hazardly through the decades along various initiatives and practices led by Western International Financial Institutions, development professionals and agencies. Development aims and goals were headed up by a number of schools of thought including the structuralists and dependency theorists of the 1960s, the modernisation and basic needs approaches of the 70s, through to the Neoliberal structural adjustment programmes of the 1980s (the so-called lost decade of development). The 1990s and into the 21st century, against the backdrop of the growing Globalisation phenomena, have heralded what is being called a more alternative and participatory approach to development theory and practice, based on a more human development and rights based approach. Scholars such as Robert Chambers have brought to the fore the importance of participatory methods to the development field, advocating methods such as PRA (Participatory Rural Appraisal) which places a greater emphasis on the role of local people in defining and solving their development problems, a realisation that villagers have a greater capacity to map, model, observe, quantify, estimate, compare, rank, score and diagram than outsiders have generally supposed them capable of (1994:1255). Despite these efforts to give development a human face many argue that such forms of so-called alternative development remain undistinguishable from the mainstream and have arguably merged, The problem is that there is no clear line of demarcation between mainstream and alternative alternatives are co-opted and yesterdays alternatives are todays institutions (Nederveen Pieterse. 1998:349). As development has crawled through the 1990s and into 2000 the gusto and courage of the post-development thinkers has merely been fuelled. Not happy with mainstream development or the alternatives it offers, post-development poses the ultimate challenge, to find not an alternative development rather an alternative too development. With its provocative statements and voice of certitude post-development challenges every development workers mind. The following discussion will draw out some of the key challenges posed by post-development including case studies and examples of development failure. Critique of these challenges will be given throughout. The collapse of the World Trade Organisation (WTO) summit held in September 2003 in Cancun marked a significant moment in time and provided a stark reminder that mainstream development is not working. At the summit unfair trade rules were met with resistance from more than 71 developing nations who refused to accept the forced agenda set by the rich nations. The result : the collapse of the talks. The failure of the rich nations to accept and negotiate resistance and the subsequent abandonment of the summit, marks what so commonly occurs within such agreements, that of Our way, or the highway! . The very same global advocates of human and economic development as a good for all showed themselves to be the very same hypocritical powers keeping developing nations trapped in crippling poverty through unfair and exploitative global trade regulations, For the developing countries, membership has not brought protection from abuses by the powerful economies, mush less serve as a mechanism of development (Bello. 2003:2). The Cancun Development Round marks a prime example of the ever apparent reality which post-development thinkers so explicitly oppose the hegemonic global dominance of the Worlds super-powers dictating progress and development based only on their terms. It is this profits before people (Kernaghan. 2001:64) attitude based on Neoliberal economics and neoclassical development theory, to which post-development is so strongly against. Mainstream economic development policies touted the world over based on economic reform, lowering of tariffs and trade liberalization in the Southern nations has been heavily criticised for its failure. Such failure has been marked by growing resistance across developing nations who are increasingly dissatisfied with World Bank and IMF Neoliberal prescriptions, and who are calling for a different development. A recent example of this cited in The Guardian, in which Lula Da Silva the elected Brazilian President states that 76% of Brazilians had voted against the current free market economic policy and in favour of a new model of development (The Guardian. 2002:14). The above example of the Cancun talks marks an entry point into a discussion of the challenges posed by post-development. Nederveen-Pieterse describes post-development as a Radical reaction to the dilemmas of development (2000:175), however whether it really is a radical standpoint is debatable, rather is it a more realistic and common-sense view from which to approach the fai de of development of the last 40 years, as the title quote from Esteva remarks In Mexico, you must be either numb or very rich if you fail to notice that development stinks (1987:1351). Post-development critiques the core basics of mainstream development theory taking overt positions on the problematisation of poverty, the portrayal of development as weste rnisation and critique of modernism and science (Nederveen- Pieterse. 2000:175). Drawing on the work of Michel Foucault on power, truth and knowledge many of the post-development thinkers challenge the imperialist, western and dominant form which, they argue, development has taken, believing that the development project was merely a form of neo-colonialism used to maintain the rich nations dominance and the poorer nations subordination, Systematic, empirical investigation of historical, geographical, and demographic conditions engendered the modern human sciences. Their aim for Foucault, was not human emancipation, but the making of docile yet productive bodies (Dreyfus Rabinow. 1983) (In Peet Hartwick. 1999:130). One of the most significant challenges posed by post-development is towards the use of modernism and technology as a development good for all. The neoclassical understanding of development draws on the lure of modern society to encourage the systematic modernisation of developing nations along western developmental paths in which industrialization, technological advance and utmost modernity can be fully embraced. For post-development thinkers, such as Illich, modernization means mass commodification and the rise of global products, a type of modernization which is suited more to the market needs of rich nations. In advocating the rise to modernity, Illich believes that a state of mind is engendered within developing nations, a state of mind which convinces them they are underdeveloped, Underdevelopment is the result of rising levels of aspiration achieved through the intensive marketing of patent products (1997:97). Thus for Illich poverty becomes planned, a scam to force developing nations into an unfair globalized economy producing foreign products for the global market and to, as Illich provocatively puts it surrender social consciousness to pre-packaged solutions (1997:97). And what of the impact the presence of such foreign firms and products have on developing nations? The impacts according to post-development, are only too apparent from the high levels of industrial pollution and environmental degradation to the use of sweatshop labour in the manufacture of global goods. A recent example in the UK press highlights the adversity of these impacts only too well as the largest Coca Cola plant in India is accused of putting thousands of farmers out or work by draining the water that feeds their wells and poisoning the land with waste sludge that the company claims is fertiliser (The Guardian. 2003). The plant employing only 141 people has been condemned by the charity ActionAid as an example of the worst kind of inward investment by multinational companies in developing countries (The Guardian. 003). In the face of such modern catastrophe and technological disaster, such as that of the big D Development Dam projects of the last two decades (including the Indian Sardar Sarovar Project in which over 200,000 people have been displaced, 56% of whom are tribal people (Kurian. 2000:843)), the post-development thinkers call on tradition, self-sufficiency and locally based forms of appropriate technology to resist, challenge and provide alternatives to the domina nt ideologies of modernism touted by global technocrats. The well documented work of Norberg-Hodge writing on Ladakh in the trans-Himalayan region of Kashmir, highlights the importance post-development theory places on traditional ways of life as a means to provide alternatives to development and challenge modernity. Writing on Ladakh, Norberg-Hodge notes how life has changed since external development forces have become increasingly significant in Ladakhi life, When I first lived among Ladakhis in the early 1970s, they enjoyed Peace of mind. The pace of their lives was relaxed and easy. An important element in this stress-free lifestyle was the fact that they had control over their own lives. Over the last thirty years however I have watched as external forces have descended on the Ladakhis like an avalanche, causing massive and rapid disruption (2001:112). She writes of the self-sufficient life which was led before development intervention, and as Rahnema and Bawtree She feels that western society has much to learn from the traditional lifestyle of the Himalayan people of Ladakh (1997:22). However, this challenge to modernism and technology and its call to more traditional ways of life does not go un-criticised. The post-development school is indeed heavily criticised for its over-romanticisation of the past, which some argue serves to artefact people and cultures, as Corbridge writes Post-development romanticises the soil cultures of the social majorities and provides poor empirical documentation of its claims (1999:145). Post-development theory also falls weak in its challenge to modernism and technology in its failure to recognise the liberating effects they may have, for example the use of cyber-technology by the Mexican Zapatistas in gaining international support and recognition, or the Kyapos use of video cameras and planes to defend their culture and ancestral lands in the Brazillian rainforests (an example cited in Escobar, 1995, implying the somewhat contradictory nature of the scholars arguments). One of the key criticisms of the post-development challenge to modernism and technology is that many believe they do not suggest adequate alternatives and merely rely on a glorification of the local, as Nederveen-Pieterse comments on the work of Norberg-Hodge, What is the point of declaring development a hoax (Norberg-Hodge. 1995) without proposing alternatives (2000:188). A further significant challenge presented by post-development is that towards the all encompassing concepts of global good, an example of which is sustainability. Since the early introduction of the concept in the Brundtland Report and its increasing prominence through international summits such as the 1992 Rio Earth Summit, Sustainability and sustainable development have become key buzz words within the development field heralded as a more integrated development goal in terms of achieving long term social, economic and environmental goals for present and future generations. However, as with many initiatives it appears that as increasing numbers of development agencies and organisations jump on the sustainability band wagon, it is proving to be another development good based merely on rhetoric. Indeed misuse of the concept has resulted in it becoming seen as another hegemonic Western discourse. An example of this can be seen in the number of inappropriate environmental projects which have sprung up in developing nations as a result of western use of sustainable development as a powerful interventionist tool. The Global Environment Facility (GEF) in association with the World Bank has been guilty of this, using un-realistic Eco-development projects in India to conserve the environment and create National Parks for Tourist purposes. The projects, carried out with little consultation from local people have resulted in the displacement of indigenous peoples from forest lands. The projects are imposed on the local communities by foreign environmentalists whose initiatives go against the basic livelihoods of those who depend on the forest resources for survival. The use of grand theory and concepts such as sustainable development again become what the post-developmentalists are so against, the imposition of development from centralised, distant bureaucracy which make decisions for communities they have never consulted (Source:2). Esteva writes on sustainability, .. in its mainstream interpretation, sustainable development has been explicitly conceived as a strategy for sustaining development, not for supporting the flourishing and enduring of an infinitely diverse natural and social life (1992:16). As the above example of sustainability highlights, post-development thinkers challenge the use of grand meta-narratives which are so commonly used within mainstream development paradigm. One of the main critiques of post-development is that it offers no alternative, it merely rejects current practice. Schurrman (2000) comments on the loss of central paradigms in development and poses the questions as to whether post-modernism, post-development and globalisation are capable of offering new and exciting paradigms? What Schurrman fears is that perhaps they are not, and as old paradigms are lost, new ones remain absent (Schurrman. 2000) The post-developmentalists would challenge this significantly, as Escobar points out By now it should be clear that there are no grand alternatives that can be applied to all places or all situations (1995:222), reiterated by Foucault who believed that all global theories such as modernization theory, Marxist mode of production theory, or world systems theory, to be reductionist, universalistic, coercive and even totalitarian (Peet Hardwick. 999: 132). These concepts and theories constructed within a western world view are strongly rejected as they challenge the scale at which mainstream development theory and practice operates, calling for more localized, grassroots-specific, bottom-up approaches. The ultimate challenge posed by the post-developmentalists to mainstream development is that of its failure, as Sachs famously puts it, The idea of development stands like a ruin on the intellectual landscape. Delusion and disappointment, failures and crimes have been the steady companions of development and they tell a common story: it did not work (1992:1). From the widening of inequality to the increased spread of HIV/Aids post-development theorists condemn mainstream development to failure. Examples such as the work of Ferguson (1994) on development failure in Lesotho based on rural development called The Thaba-Theska Project funded by the World Bank and Canadian International Development Agency in 1974, or even recent pieces in the Press such as an interview with Michael Buerk in the January edition of the Radio Times, in which he comments on his visits to Ethiopia in 1984 compared to a recent visit in 2004 in which he states The fact is that there are twice as many people hungry in Ethiopia today as there were in 1984 (Michael Buerk. 004:153), all point to failure. Despite the stark truth of development failure in some cases, others argue that such a negative standpoint and utter rejection of development does not ring true across the whole of the developing world, where examples of success and progress have been made. Corbridge offers the following critique, Post-development gives no hint of the extraordinary accomplishments that have defined the age of development, or of the historically unprecedented increases in life expectancies for men and women that have been achieved since 1950 (In India, life expectancies at birth increased for men from 46-60 years between 1965 and 1990, and for women from 44 to 58 years (Corbridge. 1999:145). Criticised for their generalisation of development, overtly pessimistic view points, romanticisation, unproblematised view of social movements and a complete rejection of development, post-developmentalists have themselves not preceded unchallenged. Indeed their tendency to deconstruct rather than reconstruct and the absence of alternatives does make many wary of the fruitfulness of such a standpoint (see Nederveen-Pieterse 2000). However, the beauty of post-development lies not in its answers but in its lack of answers. Post-developmentalists challenge the global super powers and International Financial Institutions such as the World Bank and IMF; they challenge civil society to resist, in similar ways to those of the Mayan indigenous population who through the rise of the Zapatistas have appealed for an end to 500 years of oppression and 40 years of development (Esteva. 994:302) and who call for greater recognition of indigenous rights; they call on NGOs, development Agencies, charities and development practitioners to rethink the way they operate, to question and to challenge the work they are doing; they challenge not only Western scholars but also those of the Third World, in particular on what Peet and Hardwick call Intellectual Dependency Theory (1999:137) a challenge to Third World scholars to move away from the dominant ideologies of Western discourse towards more critical and creative thinking on th e issues facing developing countries; they also pose challenges to themselves, to their body of knowledge which indeed does not provide answers. However, ultimately post-development challenges us, both our mind set, ways of thinking and assumptions. To conclude it must be stated that despite its obvious drawbacks, post-development successfully provides a series of provocative challenges to mainstream development paradigms, indeed Corbridge sums up the power of post-development and the opportunity it provides for future change, Post-development keeps the raw nerve of outrage alive post-development thinkers force us to confront our own prejudices about the agendas of development and the shocking failure of some aspects of the development project. They also provide a human touch that is too often missing in development studies (1999:143).

101 Time Management Strategies For Marketers - CoSchedule

101 Time Management Strategies For Marketers As content marketers and bloggers, we all feel like we could use some better time management strategies from time to time. In fact, one of the biggest takeaways we learned from our 2016 Better Marketer Survey  is that our readers are short on time. As we dug deeper into the data, it wasn’t hard to see why. Most of our audience is working alone or with a small team, and usually without all the resources required to create all the content they need. The majority of respondents are also fairly new to content marketing and blogging, with 63% reporting they have two years of experience or less. Put that all together, and it sounds like a recipe for burnout. If you’re feeling overwhelmed, however, there are ways you can get in control to tilt the odds for success back into your favor. By optimizing your workflow, leveraging the right tools, and learning to focus on the tasks where you can make the most impact, you can make your life as a content marketer less stressful and more satisfying while increasing your productivity. Here are 101 marketing time management strategies to help you better manage your time, work more efficiently, and get back in control of your content. Table Of Contents Content Strategy Ideation Organization And Project Management Team Communication Content Writing Graphic Design Search Engine Optimization Social Media Email Content Calendar Management Analytics And Reporting General Productivity 3 Content Strategy Time-Saving Tips 1. Think MVP (Minimal Viable Product) When planning your content strategy with limited time, ask â€Å"what is the minimum amount of resources and effort each project will take to be successful?† This isn’t the same as cutting corners, however. Think of this process as a way to mentally cut your content down to what’s most essential, and leave anything that isn’t critical to your success on the cutting room floor. 2. Refresh And Republish Instead of creating completely fresh content every time you sit down to write a post, consider identifying older content you can potentially update and refresh. To do this, use Google Analytics to find your top performing posts. Then, search the primary keyword for each post and see what content is currently ranking on the first page of Google. If those posts have newer or more relevant content than your own post, double down on revising your posts to be even better than theirs. Then, republish it as a new post, or an updated version of the original. This strategy can help you save time by leveraging what you already have, rather than having to come up with something new from scratch. Plus, it can also be a good way to get more mileage out of posts you know already resonate with your audience. Recommended Reading:  How To Breathe New Life Into Old Blog Posts 3. Create A Quick Reference List Of Content Curation Sources Identify influential sources in your niche you can lean on for content curation and put them into a list you can refer back to later. That way, you won’t have to hunt for sources later. Back To Table Of Contents 15 Content Ideation Time Management Strategies 4. Use Google Trends Use Google Trends  to quickly gauge searcher interest in a given topic. It’s a free and easy-to-use tool that shows how frequently a given topic is getting searched, which is extremely helpful for validating the worth of your content ideas. 5. Find Top-Performing Content Fast With BuzzSumo If you’re ready to move up to a paid tool, BuzzSumo  is a handy tool for surfacing the top performing content on a given topic. It can help you not only determine what’s popular, but also show you what kind of competition you’re up against. 6. Don't Forget About Google's Keyword Planner While it’s primarily a PPC tool, Google’s own Keyword Planner  needs to be mentioned here as well. It’s useful for both generating keyword ideas as well as estimating the potential search traffic they could deliver to your post. 7. Leverage Your Competition Manually review the most recent posts from your top competitors blogs and websites. Look at what they’re doing to get an idea of what topics are hot at the moment. Keep your eyes open for new information that you could add to a post, that no one else has (yet). 8. Try Portent's Content Idea Generator Portent’s Content Idea Generator  is good for at least getting the gears in your head turning on ideas you can use quickly. Hubspot’s Blog Topic Generator  is similar and worth checking out, too. 9. Use Feedly Use Feedly  to quickly keep tabs on recent news and blog posts. It's similar to what Google Reader used to be, pulling the latest posts from different sources you find in one place for easy reading. 10. Take Notes And Keep Them Organized Save time trying to find where you’ve kept all your inspiration filled notes with a tool like Evernote. Alternately, something like Google Keep  or any note taking app can also work well. 11. Set Time Limits On Brainstorming Sessions Brainstorm (with a time limit). Have your team write down as many ideas as they can think of in five minutes. Put them on notecards and then post them on a board. Go through them all and rank each idea a 1 (so-so) to 3 (brilliant). Discard anything that is not a unanimous 3. This process can easily yield dozens of strong ideas in under an hour. Recommended Reading:  4 Simple Brainstorming Techniques That Will Help You Write Killer Content 12. Use A Voice Recorder Or Mobile App To Take Notes On The Go Inspiration can come anywhere and at any time, so make a habit of recording your ideas whenever they pop into your head. Use a voice recorder or note-taking app on your phone, or consider carrying around a notepad in your work bag. 13. Subscribe To E-newsletters In Your Niche Or Industry Subscribe to quality industry email newsletters. Following thought leaders in your niche is a lot easier when you’re getting their latest information sent directly to your inbox. 14. Use Internal Search Terms To Generate Content Ideas Use internal site search analytics  to see what people are looking for on your site or blog. This can be a fast way to identify gaps in your content (if people are looking to you for something you don’t have content for, then you know it’s time to get to work). 15. Use Downtime To Research On Your Phone Turn wasted time into productive time by using your phone to research while waiting in shopping lines, riding public transit, or anywhere else you find yourself waiting (and when and where it's acceptable to use your phone). Smarter #Marketer Tip: Waiting in line at the store? Use that time to research on your phone....16. Audiobooks Are Your Friend Listen to audiobooks related to your professional development on your commute instead of music or the radio. You'll pick up information you can then put to use when you get to the office. 17. Use Google Analytics To  Identify Past Success Use data from Google Analytics to predict success and avoid wasted time on things that won’t work. For example, if you notice that several posts on a given topic aren’t performing well, consider whether the issue is the quality of your content, or the possibility that the topic simply doesn’t have an audience. If you know certain topics resonate with your readers and you have the data to prove it create more content like that. 18. Create An Ongoing Idea List Maintain a list of content ideas you can pull from in the future. Keep it in a Google Doc, spreadsheet, or Evernote doc that your entire team can access and edit. Then when it comes time to write a post, you can just pull an idea off the list instead of wasting time wondering what to write about. Back To Table Of Contents 6 Organization And Project Management  Strategies 19. Use A Shared Meeting Calendar Use a shared Google Calendar to make sure everyone on your team has visibility on meeting times, events, out-of-office requests, and so forth. 20. Use Consistent File Names Establish a consistent naming convention for saving files. This can make finding things later much easier. Recommended Reading:  How To Rock A Content Development Process That Will Save You Tons Of Time 21. Take On  Projects In Logical Order Group similar projects together and knock them out all in a row as sprints. For example, list all the steps required to write one blog post. Then, run through them starting at the top. 22. Follow  The 80/20 Principle Also known as the Pareto Principle, the 80/20 principle  is the idea that 80% of your output will come from 20% of your effort. To leverage this for your work, start by eliminating or delegating menial non-essential tasks that don't directly impact results. The more you focus on things that directly affect productivity, you'll get more done with less effort. 23. Be Realistic About What You Can Do Prioritize projects and only take on the ones that are within the scope of your resources and stand a reasonable shot at success. 24. Set Goals For Every Task Set progress goals for every project and stick to them.  They'll keep you focused on your end result and help you build discipline. Back To Table Of Contents 7 Team Communication  Strategies 25.  Use A Messaging App Instead Of Email For inner-office communication, a tool like HipChat  or Slack  can improve efficiency while keeping your email inbox uncluttered. 26. Use Screenshots For Design Edits A picture is worth a thousand words. If you need to suggest design edits, sometimes sending screenshots is the best way to go. Use a tool like Skitch, SnagIt, iAt Home, or Awesome Screenshot  to take screen captures with notes and arrows. 27. Know When To Delegate If someone else can do a task 80% as well as you can, and you don’t have time to do it yourself, consider handing it off to another team member (so long as they do have time). 28. Know When To Ask For Help Ask for help before you’ve wasted too much time trying to figure something out on your own. There’s something to be said for showing initiative and trying to learn things yourself, but if you know that someone on your team already has that knowledge, just ask. You’ll get more done that way. Strategy #28: Save time by asking for help sooner. #ContentMarketing29. Keep Shared Files Easily Accessible Make sure everyone knows where to access files and documents your team will use. This sounds basic, but if you’re using multiple resources to store files, you’d be surprised how easy it is for things to get out of control if your team doesn’t know what goes where. 30. Establish Team Workflows Establish consistent workflows to save time wondering what each person should be doing at each step of a project. To do this: Get your team together to create a list of every step required from each team member to complete the project (be sure you do this before doing any of the actual work itself). Put those steps in order. Identify who will be responsible for each piece. Have each person notify the next team member when it's ready to hand off. By the time you're done, you'll have an effective workflow template you can follow on similar projects in the future, refining the process as you discover what works and what doesn't. 31. Set Up Regular Times To Check In Schedule regular touch-point meetings and stick to them. This removes excuses for not having time to communicate, or for team members not sharing vital information. Back To Table Of Contents 17 Content Writing Productivity Strategies 32. Use Outlines Create an outline  for every blog post. Knowing the structure of your post will help you write it quicker and create more focused content. 33. If You're Overwhelmed, Do Better At Doing Less Think less is more. If you’re feeling overwhelmed trying to create too many posts, try putting more effort into fewer posts. Sometimes having greater focus on fewer things can help rein in some of that stress. Recommended Reading:  How To Blog With As Little As Possible 34.  Avoid Pursuing Perfection Don’t cut corners on quality, but do avoid chasing perfection. There will always be things you want to change, so focus on shipping and know when to call your work finished. 35. Edit Your Own Work First Edit your own work first before passing anything off to a proofreader or copy editor. It’ll help save them time and better endear you to staying on their good side. 36. Write Big Posts One Piece At A Time Break down long-form pieces into smaller chunks and work on one section at a time. This can help you work faster by focusing on smaller portions of the overall post, instead of getting overwhelmed by the full scope of your idea. 37. Set Deadlines Deadlines. Set them  and meet them. Don’t let your work stretch to fill an excessive amount of time. 38. Hire Help Don’t have time to do everything yourself? Consider hiring freelance help if you can. 101 tips to save time with #blogging and #contentmarketing (spoiler alert: use ).39. Turn Coworkers Into Blog Contributors Leverage internal experts within your organization. If you’re working in-house with a business, get buy-in from other team members with unique expertise who may be able to help contribute blog posts. 40. Schedule Writing Work Blocks Block off focused and uninterrupted time to write. Distraction-free word processors like FocusWriter  or WriteBox  can be useful if you really want to get serious about eliminating distractions. This way, you can get more writing done quickly without getting sidetracked. 41. Forget Word Counts Don’t waste time worrying about hitting specified word counts (unless you’re working with an editor or manager who is adamant about a specific word count). The right length for your post is however long it takes to write a full and comprehensive piece of content on your topic. Usually, that’s going to mean a substantial length (1,500+ words), but not always. 42. Consider User-Generated Content Consider accepting unsolicited reader submissions or create a section where readers can write their own blog posts on your blog (this will not necessarily work nor be appropriate for every blog). Moz’s YouMoz Blog  is a great example of how to do this well. 43. Solicit Guest Bloggers Having some extra help from reputable contributors can make it much easier to get authoritative content on your blog with less effort from your own team. 44. Use Hemingway Want to know if your writing sucks? The Hemingway app  will let you know, and help you identify ways you can make your writing clearer. It’s available for both Mac and PC, and is worth its $10 price tag, especially if you don’t have an actual copy editor or proofreader. 45. Use Use a tool like to schedule your blog publishing and associated social media content ahead of time. Is this a shameless plug? Absolutely, but there's a reason why we build our product. (If you haven't tried it yet, get your 14-day free trial here.) 46. Write Headlines With The  Headline Analyzer Use the  Headline Analyzer  to quickly write and score headlines. This makes it easy to write lots of headlines fast and choose the best option. 47. Use The Skyscraper Technique Use the skyscraper technique  to quickly identify top performing competitor content you can improve on. This simply involves looking at the top-ranking content on your given topic or keyword, identifying what those posts are missing, and then making sure your own content fills those gaps. That could mean including information those other posts are missing, or different types of content they lack (for example, embedded videos or infographics). 48. Repurpose, Repurpose, Repurpose Repurpose content from your blog posts for other content. Borrow blog post copy for your email newsletter. Turn several related blog posts into an ebook. These are just a couple ideas, but if you need more inspiration, here's over 50 ideas for repurposing content. Back To Table Of Contents 5  Graphic Design  Time Management  Strategies 49. No Designer?  No Problem. Don’t have access to Photoshop? Or, does your team lack a dedicated graphic designer? In either case, tools like Canva, Infogr.am, and Piktochart make it easy to create high-quality images and infographics you can use for your content. While the above tools are definitely useful, sometimes you really need dedicated design and image manipulation software to get the job done right. If cost is the only thing stopping you from investing in Photoshop, consider GIMP. It’s a free and open-source alternative to Photoshop that, while lacking certain features, still packs an incredible amount of power. This isn't necessarily a time-saving tool, per se, but if you've been putting off creating graphics because you don't have budget for the right tool, now is the time to stop wasting time with excuses and start getting down to work (the payoffs for having quality graphics are worth it in the long run). 50. Reuse Graphics Don’t forget that one graphic can potentially be used multiple places. For example, try remaking blog header graphics in multiple sizes for email newsletters and social media posts.  If you have certain graphic elements you use frequently, consider storing them all in one place where they can easily be accessed. 51. Use Keyboard Shortcuts Learn Photoshop keyboard shortcuts. The time you spend internalizing them into your workflow will save you tons of time in the long run. 52. Use Image Templates Create your own Photoshop templates for common image sizes and formats you know you’ll be using often, or find downloadable templates online (a quick Google search should yield plenty of options). 53. Give Your Designer Direction  Before They Start Working If you're a writer or blogger working with a graphic designer, give them an idea of what you want for graphics before they get started. This can help avoid confusion and having to redo images when the results don't match expectations. For graphic designers, this same advice applies in reverse. Ask your writer what they want up front. Even if they can't quite communicate exactly what they're looking for, opening up the discussion early in the collaborative process can be an immense time saver later on. Back To Table Of Contents 4 Search Engine Optimization Time Management  Strategies 54. Use Yoast If you’re using WordPress, use the Yoast plugin  to quickly check your on-page SEO and make sure your title tags and meta descriptions are well written. 55. Get An SEO Platform If you have a budget, use a dedicated SEO software platform to manage your search engine optimization efforts. Moz  and Raven Tools  are both affordably priced and fully featured options that will cover most of your bases. Backlink tools like Majestic  and Ahrefs  are well worth a look as well. The benefits to using these services are that they keep your data stored in one place and make performing SEO tasks much quicker, easier, and more effective. 56. Leverage Local SEO Tools To Quickly Clean Up Citations If local SEO  has any relevance to your business (meaning, if you have a brick-and-mortar location and rely on search to drive foot traffic), Whitespark  and Moz Local  are two great tools to help speed up a lot of menial tasks that can otherwise eat up valuable hours of your time. 57. Use Screaming Frog Fixing broken links on your blog or website can be time-consuming. However, it helps to pay attention to them and get them fixed as an ongoing practice, rather than letting them accumulate and having to fix a ton of them all at once. Use Screaming Frog  to quickly crawl your site and identify URLs that return a 404 status code. Back To Table Of Contents 15 Social Media Time Management Strategies 58. Use One Tool To Manage All Your Accounts Manage all your social media accounts with one tool. , Hootsuite, and Buffer  are all valid options for this task. By logging into one dashboard instead of multiple sign-in pages each time you want to schedule social media updates, you can easily save an enormous amount of time. 59. Automate Social Media Publishing Automate (some) of your social media content. You’ll want to continue adding a personal touch to your social media posts, but filling your social queues with pre-scheduled content can help you maintain a consistent presence with less active effort and attention. 60. If Something Isn't Working, Stop Get rid of platforms that aren’t working. Focus more effort on fewer channels. You’ll likely be less stressed and more successful. 61. Use IFTTT To Automate Social Media Tasks Use IFTTT  to automate recurring tasks. It's a cool tool that uses "recipes" to automate processes (and has applications that go way beyond just social media). Here's a good video tutorial on how it works: 62. Use Rapportive To Gather Social Profiles From Emails Get an email from someone and want quick access to their social profiles? Use Rapportive. It's quicker and easier than manually searching for their Twitter handle. 63. Use Tomoson To Find Influencers On Social Media Use Tomoson  to find influencers and brands who might be interested in your product or service. Rather than manually searching for experts on a given topic, it can make finding prospects for this kind of social media outreach quicker and more effective. 64. Avoid Social Media When You're Supposed To Be Doing Something Else Eliminate distractions from your personal social media when you’re working. You’d be surprised how much time you might be wasting checking your Facebook updates or Twitter stream. 65. Respond To Comments, But Keep It Short And Sweet Expedite social media response times. If you don’t have the time or energy to write up a thoughtful response to a social media post, sometimes a simple â€Å"Thanks for reading!† is enough to let someone know you've heard their feedback. 66.  Turn One Idea Into Multiple Posts Try writing a few different versions of a single post. Then, schedule those posts out throughout the week. Now one idea for your post has become three. 67. Use Twitter Lists Use Twitter lists to quickly follow influencers on specific topics. You can either create lists yourself to monitor accounts based on topics or interests, or simply search Twitter for lists on topics you’re interested in and follow them. #TimeSavingTip Use Twitter list to quickly follow influencers and topics without the noise....68. Use Storify Use Storify  to round up tweets and social media posts quickly. 69. Use Social Media Engagement Metrics For Instant Content Feedback Use social media engagement numbers as a rapid-fire real-time indicator of which content is most popular. 70. Use Hashtags Smartly Leverage hashtags to easily increase exposure. Be sure to choose hashtags that directly relate to your brand, blog, or content. This may seem basic, but it's an often overlooked tactic that can help your posts gain more visibility quickly. 71. Write Posts In Bulk Write social media posts in bulk and schedule them out at least a week or two in advance. 72. Use Followerwonk Want to analyze your Twitter performance and find influencers quickly? Try using Followerwonk. It’s free, fast, and easy to use. Back To Table Of Contents 6 Email Marketing Time Management Strategies 73. Use A Real Email Marketing Platform If you don’t have access to a developer who can build you custom email newsletter templates, then use a platform to manage email like MailChimp  or Campaign Monitor  (at , we use Campaign Monitor). They’re easy to use and make email marketing management a lot more efficient than going without them. 74. Resend Past Email Newsletters Instead of writing a whole new email, try taking your last email and sending it to anyone on your list who didn’t open it the first time (be sure to wait about a week first). 75. Use Pop-Up Forms To Build Your Email List Use automated pop-ups on your blog or website to build your email list fast and convert readers into email subscribers. SumoMe's Scroll Box is an  easy to use tool for this task, available in  both free and premium versions. 76. Give Away Free Bonus Content  To Drive Email Signups Use content upgrades to entice more email subscriptions. A content upgrade can be defined as a gated piece of content (like an infographic, ebook, video, course, or something else) that requires readers to exchange their email address in order to access the content upgrade. One piece of content can equal tons of subscribers, making this a highly efficient way to grow your email list. 77. Automate Email Follow-up With Boomerang If you need to automate a lot of messages for one-on-one email outreach, use Boomerang  to follow up on emails. 78. Check Email At Set Times (Instead Of Literally All The Time) When it comes to your own work email, set time aside to check your email rather than checking it every five minutes. If you took our suggestion of using HipChat or Slack for inner-office communication, you’ll probably notice the amount of email hitting your inbox dropping substantially, too. Back To Table Of Contents 3 Content Calendar Time Management  Strategies 79. Use A Content Calendar If you aren’t already, use a content calendar. Any kind of calendar. We’re going to plug ourselves quick here, but using can help save tons of time here. 80. Schedule Content Two Weeks In Advance Keep your calendar full for at least two weeks out into the future. You’ll get more done when you’re not stressed over hitting immediate deadlines. That may mean having to work a little extra hard at first to build up enough content to fill your calendar, but once you've gotten past that initial effort, you'll find yourself freer to strategize and work more efficiently. 81. Consistency Is Key Be consistent when updating your content calendar. Build habits that save time and your calendar  will, in turn, help you save time elsewhere in your workflow. Back To Table Of Contents 3 Analytics And Reporting Time Management Strategies 82. Use Pre-Built Google Analytics Dashboards If you’re not skilled in configuring Google Analytics yourself, get more utility from it faster using pre-built dashboards. That saves you time from having to learn how to configure everything correctly yourself  while making sure your dashboards are designed to be useful. 83. Use SumAll To Gather Social Media Data Use SumAll to automatically generate data-driven social media performance reports (note that as of March 1, 2016, this is a paid-only tool). 84. Set Up Automatic Reporting In Your SEO Platform If you’re using Moz, Raven Tools, or any other content marketing and SEO platform, use it to set up automatic reports delivered to your email. That way, you can provide reports to management quickly without having to build out reports manually. Back To Table Of Contents 17 General Time Management Strategies 85. Track Your Time Time tracking tools like Toggl  work well for this. Or, just use the timer on your phone. Over time, you’ll get a better idea of how much time tasks generally take, and be better able to plan accordingly in the future. 86. Use The Pomodoro Technique Try using the Pomodoro technique  throughout your workday. In short, it’s a productivity method where you work in 25-minute bursts, separated by break periods. 87. Take Timed Breaks Even if you don’t use the Pomodoro technique, taking timed breaks is still highly advisable. Your brain simply can’t fire on all cylinders around the clock. Taking breaks can actually increase your #productivity.88. Use A Password Manager Use a password manager to save time entering passwords or recovering passwords you’ve forgotten. 1Password  and LastPass  are both solid options. 89. Don't Fear Failure Fail fast and move on. Don’t let the fear of failure stop you from trying something new that may work better than what you did yesterday. 90. Don't Waste Time On Things That Aren't Working Likewise, if something isn’t working, either figure out why it’s not working or ditch it and move onto something else. Bias yourself toward action and don’t waste time overanalyzing things. 91. Avoid Multitasking Stop multi-tasking. Studies show you’ll get more done, more quickly if you focus on one task at a time. 92. Use Checklists Create task checklists to keep yourself on track. Wonder what makes checklists such an effective tool for getting things done? Read The Checklist Manifesto. 93. Never Start A Meeting Without A Plan Plan your meetings and set timers. Never go into a meeting without an agenda or a goal that the meeting is intended to help achieve. 94. Only Use Tools That Show Real Benefits Use tools and software services that deliver demonstrable productivity benefits. Ditch any that don’t. That saves time and money all in one fell swoop. 95. Take On Your Toughest Task First Thing In The Morning Do your most dreaded task first thing in the morning, whatever that task may be, to free up mental space to take on the rest of your day. 96. Get Enough Sleep Sleep. You’ll get more done, more quickly if you’re well rested. Working when you’re tired leads to diminishing returns over time. 97. Don't Say Yes To Everything Learn how and when to say no. While it’s often tempting to take on every opportunity and task handed your way, you don’t want to burn yourself out by taking on too much. Be realistic about what you can accomplish with the time you have. Simple but true: Saying yes to everything leads to #burnout. Learn how to #sayno. #productivity98. Leave Enough Time For Small Setbacks Leave a time buffer when estimating time for tasks to account for unforeseen hang-ups. This can help avoid missing deadlines. This is important because missed deadlines have a way of snowballing by pushing out timelines on other projects. 99. Make Time To Plan Set aside time for planning. It takes time, but it also saves time in the long run. 100. Use Friday Afternoons For Routine Maintenance Tasks Plan your least mentally intensive tasks for Friday afternoon when you’re probably drained. That way they won’t distract from more important work but they’ll still get done. Recommended Reading:  What To Do On The Weekend To Increase Your Productivity On Monday 101. Plan Tomorrow, Today Use the end of one day to plan what you’ll do at the start of the next day. You'll likely be feeling drained around the end of your day, which makes it a good time to write up a simple list of priorities for your next morning. You'd be surprised how much time this can save while making optimal use of your energy levels. Back To Table Of Contents That Wraps Up All 101 Marketing Time Management Strategies Developing better habits isn’t always easy, and odds are, it’s going to take a little while before you can start applying the majority of these time management strategies to your content marketing workflows. However, all of these items are things you can start doing today, taking things one small step at a time until you’ve established more efficient and productive ways to work. Are there any tips, tools, or tricks we’ve missed? Sound off in the comments below, and thank you for reading!

Anglo-Saxon Chronicle Essay Example | Topics and Well Written Essays - 1500 words

Anglo-Saxon Chronicle - Essay Example The title 'the Unready', or more accurately 'the Ill-Advised', given to Alfred's successor derives largely from the writer of the Anglo-Saxon Chronicle who was an admirer of Alfred and disappointed in his successor. The author seems so much unknown of the real dilemma that he has kept the then public views in his mind while writing and the chronicle is not written keeping the real circumstances in consideration Ethelred had to face in that era, but its all about the reiterate defeats of Ethelred. This is the main reason as to why Ethelred has gained nothing but only the bad reputation in history. Another reason is while writing 'Anglo-Saxon Chronicle', he has been compared to Alfred. In 1979 the year after the accession of King Aethelred, the Danish invasions, long unintermitted under Edgar the Peaceful, recommenced as their main objective was to plunder only, not conquest, and they repeatedly attacked in 981, 982 and 988. A period of peace in the middle of the tenth century, in which took place a great monastic reform and revival of learning, was followed by renewed Danish attacks, which continued throughout the reign of Aethelred the Unready and culminated in the conquest by Cnut. In 991 the Danes burned Ipswich, and defeated and slew the East Saxon ealdorman Brihtnoth at Maldon. After this incident, Aethelred realizing the eruption of law and order offered them a price of freedom of 10,000, just to bring peace and serenity in England. As the Danes had to give something in the form of desistion from their ravages, they were still allowed to stay in England. Next year Aethelred himself broke the peace by an attack on the Danish ships. Despite the treachery of A elfric, the English were victorious and the Danes sailed off to devastate Lindsey and Northumbria. In 994 Olaf Tryggvason, King of Norway, and King of Denmark, Sweyn Forkbeard united in a great invasion and attacked London. Frustrated by the bravery of the citizens, they sailed away and harried the coast from Essex to Hampshire. Now Aethelred had no option left except for paying another price of England, and once again he bought peace for 16,000 with a promise of supplies. Olaf after receiving such great favors assured Aethelred that he would never again come to England with hostile intent, an engagement that he faithfully kept. One of the reasons why he is acknowledged as 'The Unready' is he was not sure who to trust and who not to trust. For his defeat lies in the fact he trusted the wrong people. This led him towards the failure one after the next and finally the King of Denmark defeated him. The promise was made to Olaf Tryggvason, the King of Norway; Sweyn (the King of Denmark) was devoid of any kind of commitment made to Aethelred so he repeatedly attacked England in 997, 998, 999, and in 1000. These frequent attacks break down the national defense and made the Government weak. However Aethelred in these crucial state of affairs did what anyone could have done for the survival of his country, he offered the then Danes the sum of 24,000, but at the same time he ordered the slaughter of each and every Danish men who were in England, which was his greatest blunder. Such a violent behavior on part of the Aethelred made the situation worse and caused Sweyn to return for revenge and remained here for two yea rs. In 1005

International Business Essay Example | Topics and Well Written Essays - 3000 words - 22

International Business - Essay Example Operating under three main segments -- Automobiles, Motorcycles, and Financial Services, the company well-known three brands -- BMW, MINI and Rolls-Royce, goes with the popularity of company. Its BMW automobile range encompasses the 1 Series, Series 3, the 5 Series, and 7 Series, the sports utility vehicles, X3, X5, and X6 and M models, such as M3, M5, and M6. It makes available to customer cars under MINI brand and motorcycles under the BMW brand. The Rolls-Royce brands include three luxury cars, Phantom, Coupe and Ghost. With continued production assembly, services and sales subsidiaries made throughout the world, (Reuters, 2010a), BWM is undeniably a global company that can be affected by globalization. One of best proofs of a company’s attainment of its long-term health is the behaviour of its stock price in the stock market as the same could indicate achievement of wealth maximization objective (Bernstein, 1993; Brigham and Houston, 2002). This paper seeks to examine how the BMW may have benefited from the actual and potential impacts of globalization on the company by analysing the possible strategies that the company has employed. This will also evaluate the possible strategies of going forward which the company might use to response to the impacts of globalization that would be identified. The paper will discuss first the different models to understand the company’s strategies. The models include the PEST, Five-Forces and SWOT and determine whether the same are applied by BMW in its choice of strategies. The paper will end-up evaluating the present strategies used by the company and recommend what should be done by company in relation with models describe. The analysis will include using available information for BMW from its 2010 Annual Report and its websites. This paper has chosen BMW as this researcher finds interesting to see how car manufacturers could really

Single Chain Antibody Production

Single Chain Antibody Production ABSTRACT The study aimed to characterise His- and Myc- tagged scFv MFE-23 antibodies produced from transformed E.coli cultures using ELISA and immunohistochemistry assays. Anti-His, anti-myc and anti-MFE secondary antibodies were used in the CEA/PBS coated ELISA plate with horseradish peroxidase-OPD chromatic reaction for detection. Culture 1 was identified to produce MFE-His and culture 2 giving MFE-Myc antibodies. The immunohistochemistry assay confirmed the CEA binding profile of scFv MFE-Myc by the comparison between negative controls, positive anti-CEA binding reactions and 4-stage anti-Myc binding of the scFv MFE-Myc examined. The CEA specificity displayed by tagged scFv MFE-Myc can be utilised in antibody-based cancer therapy by targeting tumour antigens specifically. INTRODUCTION Cancer arises due to the defective regulation of normal cell proliferation and homeostasis. This allows tumours to possess the capabilities including self-sufficiency in growth signal, insensitivity to antigrowth signals, avoidance of apoptosis, unlimited replicative potential, sustained angiogenesis and metastasis.1 In additional to the surgical removal of tumours, conventional cancer therapies such as radiation, chemotherapy and immunotherapy have a focus in inducing cytotoxicity against malignant cells. With better understanding of the molecular biology of carcinogenesis, targeted therapies are being developed to achieve lower toxicity to normal tissues and higher clinical efficacy through disrupting pathways that contribute to the tumours proliferative advantage. Attempts are also being made in developing cancer gene therapies to compensate or repair the mutated genes. All tumours express their unique set of antigens on cell surface which can be a result of genetic alterations, upregulated self-antigens or tissue-specific antigens that can be utilized to distinguish cancer cells from normal cells. Antibodies, one of the effectors in immune response, are Y-shaped proteins that each recognizes and binds to specific antigen (Figure 1). The protein consists of two light chains and two heavy chains, with variable (Fab) and constant (Fc) domains on each of the chain. The region of the antibody at which antigen binds is referred as the Complementary Determining Regions (CDR) present at the variable Fab region. The Fc region is responsible in modulating immune response through activation of the complement cascade and Fc receptor mediated activation of effectors such as phagocytes, mast cells, neutrophils and Natural Killer (NK) cells.3 The initial approach of antibody-based cancer therapy is to tag cancerous cells as foreign and eliminate such targets thro ugh cytotoxic effectors of the human immune system. Antibodies against essential growth factors can be synthesized to sequester such molecules from further promoting tumour growth. The high specificity of antibodies to particular antigens also serves as a vehicle in delivering killing machineries to tumour cells. For example, antibodies can be directly conjugated to radionuclides, toxins or cytokines, or indirectly to the surface of liposomes carrying drugs or toxins.4 Antibody-directed enzyme prodrug therapy (ADEPT) is also available in which the antibody targets an enzyme selectively to the tumor where it converts a relatively non-toxic prodrug to a potent cytotoxic drug.4 These various strategies aim to minimize the systemic toxicity afflicted by the cytotoxic agents administered. Monoclonal antibodies are usually preferred compared to polyclonal ones, as they recognize specific epitope of an antigen and hence have greater specificity. Carcinoembryonic antigen (CEA) is first identified as a glycoprotein in the human colon cancer tissue extract and fetal gut, and plays a role in cell adhesion.5 Although CEA can also be detected in normal gastrointestinal tissue, the glycoprotein is overexpressed on the plasma membrane of colon cancer tissues.5 CEA level has also been found to be highly elevated in various cancers of an epithelial origin such as breast, lungs and pancreas.5,6 Moreover, normal CEA is localised on the luminal surface of columnar epithelial cells lining the crypts of the intestine so the glycoproteins are not directly accessible to the blood flow.6 However CEA is usually found on all sides of the cell membranes in tumours. Thus, CEA can be a useful target on cancer cells in immunotherapy using anti-CEA antibodies. The objectives of this lab practical are to introduce the applications of antibodies in cancer therapy and diagnosis through immunohistochemical staining of tissues. It also serves to familiarise students with the enzyme linked immunosorbent assay (ELISA). This is achieved by the production of MFE-23 single chain Fc antibody fragment (scFv) against CEA from transformed E.coli cultures. The scFv MFE-23 can be linked to either a His- or a Myc- tag. The unknown antibodies are characterised in the ELISA assay using appropriate detection antibodies, and the chromatic reaction between horseradish peroxidise (HRP) and OPD substrate. The scFv MFE-23 obtained by students is also used in the immunohistochemical characterisation in cryostat sections of normal and cancerous human tissues. MATERIALS METHODS E. Coli growth curves E.coli cultures were transformed with pUC119 containing either His- or Myc- tagged scFv MFE-23 (Culture 1 2) and were incubated overnight. The expression of pUC119 was controlled by the lac operon, which could be induced by either lactose or lactose analogue isopropyl-1-szlig;-D-thiogalactoside (IPTG). The vector also encoded for ampicillin resistance. The E.coli cultures were grown with ampicillin selection and 0.05% glucose. Optical density (OD) readings at 600nm were taken at 30min interval until OD=0.9 when IPTG was added to both cultures to induce pUC119 expression and hence scFv MFE-23 production. A negative control of 2xYT was set up, and all three cultures were incubated at 30? overnight. Full experimental procedures are described in Appendix 1. ELISA assay The ELISA assay aims to characterize the identities of the tags conjugated to scFv MFE-23 obtained in the supernatant of the overnight E.coli cultures. Detailed protocols of the assay can be found in Appendix 1. 36 wells of the 96-well ELISA plate were coated with the CEA antigen or phosphate-buffered saline (PBS) as its negative control. Supernatant of the overnight E.coli cultures 12 from the bacterial growth curve assay was obtained which contain either His- or Myc- tagged scFv MFE-23. The supernatant samples 12, the positive control MFE-his-myc antibodies and the negative control 2xYT growth medium were added to the corresponding wells as indicated in the format diagram in Appendix 2. Secondary antibodies rabbit anti-MFE23 polysera, mouse monoclonal anti-HIS tag (TetraHis, Qiagen) and mouse monoclonal anti-MYC (Sigma) were added to the corresponding wells (Appendix 2) to bind the primary antibodies present. Tertiary horseradish peroxidase (HRP) conjugated antibodies against the secondary antibodies goat anti-rabbit HRP (sigma) and sheep anti-mouse HRP (Sigma) in blocking solution were added to the wells (Appendix 2). OPD substrate buffer was applied to each well to detect the presence of HRP, which should give a yellow-orange product in case of positive result. HCl was added to stop the reaction when colour has developed, and the OD at 490nm for each well was measured. Immunohistochemistry assay Five glass slides, each containing 2 colonic adenocarcinoma, 1 normal colon and 1 normal liver tissue sections were fixed and processed for immunohistochemical staining. Avidin-biotin-peroxidase complex (ABC complex) was added following the application of biotin-labelled antibodies. The localisation of antigens was visualised by the formation of brown pigments, as peroxidise reacts with the diaminobenzidene (DAB) substrate. Lattices of several peroxidase molecules were formed to amplify the binding signal from the biotinylated antibody.7 A summary of the treatment given to each slide was illustrated in Table 1, and the complete protocol can be found in Appendix 1. RESULTS E. Coli growth curves Both E.coli cultures transformed with either His-tagged scFv MFE-23 or the Myc-tagged version in a pUC119 vector follow a similar exponential growth curve, as shown by the plot of OD600 against time (Figure 2). The bacterial cultures were in lag phase at t=0-90, and the log phase from t=90. It took approximately 175minutes for the cultures to reach OD600=0.9. The PBS negative controls in wells A-F/7-12 worked relatively accurately with low OD490 readings. Most readings also corresponded to the negative controls in the CEA coated wells (B4-6, D4-6, F4-6) but with 2xYT added instead of primary antibodies. However, the OD490 readings for wells C10-12 and E10-12 were higher than most readings from the negative controls. The higher OD490 readings obtained in certain wells in comparison of their corresponding negative controls indicated the presence of yellow-orange product formation from reaction between HRP and OPD substrate. These positive outcomes found in certain wells are highlighted in the shaded cells of Table Immunohistochemistry Assay Figure 4 shows the immunohistochemical binding reactions for the negative controls (slides 3-5). Slide 3 acts as a negative control for slide 1, treated with the 4-stage anti-Myc technique but omitting the primary Myc-tagged scFv MFE-23 antibody. Thus we should not be able to visualize the localization of CEA antigens due to the absence of MFE-23 binding on slide 3. Slide 4 is a negative control for slide 2, omitting the mouse monoclonal anti-CEA A5B7 antibody treatment in the 3-stage mouse monoclonal technique. No binding reaction is expected on slide 4 as well. Slide 5 was treated like slide 4 but without initial biotin/avidin blocking. As expected, the colonic adenocarcinoma tissue section of slide 3 does not display brown colouration and hence there is no binding reaction to the cytoplasm of tumour cells and connective tissues. Binding reaction to the cytoplasm of cryptal epithelium is not seen for the normal colonic mucosa, although there are strong brown colourations for a few cells in the lamina propria. The normal liver tissue on slide 3 shows some weak reaction with the parenchymal cells. Similarly, the parenchymal cells of the normal liver tissue on slide 4 do not display brown colouration and hence indicates the absence of binding reaction. On slide 5, the normal parenchymal cells are positive for binding reaction, demonstrating the presence of biotin in normal liver. Figure 5 shows the immunohistochemical binding results of slides 1 and 2. Slide 1 was treated with the 4-stage anti-Myc technique and slide 2 is the positive control treated with the 3-stage mouse monoclonal technique (See Table 1). For the colonic adenocarcinoma tissue on slide 1, strong brown colouration is present in the cytoplasm of tumour cells and the basement membrane of malignant acinar structures. Weak positive binding reactions can also be observed in fibrovascular stroma. The normal colonic mucosa of slide 1 shows strong reactions to the cytoplasm of goblet cells in the cryptal epithelium as well as a few cells in the lamina propria. The normal liver tissue shows only weak positive reactions with the parenchymal cells. Thus the scFv MFE-myc antibody was reactive with both normal colonic epithelium and adenocarcinoma, but not the biotin/avidin blocked liver tissue. The colonic adenocarcinoma tissue on slide 2 shows strong reactions with the tumour cell cytoplasm and the basement membrane of malignant acinar structures similar to the reactions seen in slide 1. Weak positive results are obtained in fibrovascular stroma of the positive control slide. As the binding reaction of slide 1 was similar to that of the positive control, this confirms the CEA reactive profile of the scFv MFE-myc antibody from the E.coli supernatant sample 2. DISCUSSION E.coli Growth Curve Both E.coli cultures followed the exponential growth curve as expected. However, the growth curve was obtained in the absence of a negative control i.e. same volume of 2xYT to be treated in the same way as the two cultures. The lack of a proper negative control means that the possibility of contamination cannot be eliminated. Thus it is unknown whether the increase in OD600 readings was partially attributed to culture contamination. ELISA Assay As mentioned in the results, the OD490 readings for wells C10-12 and E10-12 were higher than most readings from the negative controls. This might indicate contamination of these wells with CEA antigens, or insufficient PBS washing following the application of HRP-conjugated antibodies. Wells A-B/1-6 were applied with anti-His secondary antibodies and so would indicate the presence of His-tag by the production of yellow-orange product. Wells A1-3 were treated with supernatant from bacterial culture 1 and wells A4-6 with that of culture 2. As higher readings of OD490 in the positive control B1-3 and wells A1-3 were obtained compared to the negative control B4-6, the culture 1 supernatant contained the His-tagged scFv MFE-23. Wells C-D/1-6 were applied with anti-Myc secondary antibodies and so would indicate the presence of Myc-tag by the production of yellow-orange product. Wells C1-3 were treated with supernatant from bacterial culture 1 and wells C4-6 with that of culture 2. As higher readings of OD490 in the positive control D1-3 and wells C4-6 were obtained compared to the negative control D4-6, the culture 2 supernatant contained the Myc-tagged scFv MFE-23. The anti-MFE antibodies added to wells E-F/1-12 can bind to both His- or Myc-tagged scFv MFE-23. Thus binding should occur against primary antibodies from both cultures 12 and also the positive MFE-myc-his control, as demonstrated by the higher OD490 readings in wells E1-6 and F1-3 compared to the negative controls F4-6. It can be observed that the anti-His antibody gave a stronger signal than anti-Myc and was due to anti-His binding more strongly to its target than anti-Myc (Unpublished results, Kogelberg, H.). Comparing the OD490 of wells E1-3 and E4-6, absorbance of anti-Myc was slightly higher than anti-His despite the lower binding affinity of anti-Myc. Thus there might be a higher concentration of MFE-Myc in the culture 2 supernatant than MFE-His in culture 1, although technical issues like washing times, salt concentration and pH, or structural characteristics affecting the accessibility of antibody can affect the amount of binding. Immunohistochemistry Assay The results obtained from slides 1 and 2 confirm the CEA reactive profile of scFv MFE-myc antibody, as both slides displayed similar binding reaction patterns in colonic adenocarcinoma. The weak positive signal in normal liver tissue in slide 1 is likely to be caused by cross-reaction of secondary mouse anti-Myc antibodies, as similar result can be observed in the negative control (slide 3). Primary antibodies may also cross react and bind to non-target tissues. This demonstrates the possible cross-reactions with antibodies and hence the importance of negative controls to eliminate such artifactual reactivity. The strong binding to goblet cells cytoplasm in cryptal epithelium of normal colonic mucosa in slide 1 is consistent with the findings of CEA present in normal colonic mucosa.5 Although CEA level is lower on normal colonic mucosa, their presence implies that antibodies against CEA in cancer therapy may target normal cells other than malignant cells. Thus it is important to control anti-CEA antibody concentration used to avoid imposing toxicity to normal tissues yet is effective in producing a clinical response on cancer. The negative controls (slides 3-5) allowed the assessment of the level of binding for secondary, tertiary and quaternary antibodies/reagents. The slides also revealed any endogenous background material that might be confused with specific binding of primary antibodies, as well as information about the basic pathology of tissues. The inclusion of normal liver sections helped illustrate the importance in carefully controlling the specific reactions of test antibodies with potential targeting specificity for other cellular proteins. The ABC complex for detecting biotinylated horse anti-mouse antibodies can also react with biotin present in liver and give a positive non-specific result. This unwanted reaction as seen in slide 5 can be prevented by biotin blocking treatment of tissue samples. Moreover, myeloperoxidase white cells containing endogenous peroxidase can react with the DAB substrate and produce brown colouration even in the absence of ABC complexes. The normal colonic mucosa in slide 3 provided examples of such artifact. The naturally occurring bile pigments in liver seen as green/brown granules under high-power bright field microscopy can also be found occasionally. Careful interpretation of slides is required to avoid false judgments as there can be false positive reactions. In addition, it should be noted that adequate fixation of the sample is important in successful localisation of antigens present and hence an accurate representation of antigen distribution profile. The procedure helps to ensure the preservation of tissue morphology, the immobilisation of antigen and the preservation of antigen immunoreactivity. It is also important to ensure optimal fixation for an adequate permeability of the tissue to the immunochemical reagents. Other than mmunohistochemistry, western blotting and immunoprecipitation (IP) can be carried out to confirm scFv specificity. Antigens transferred to nitrocellulose membrane can be probed by specific antibodies in western blot, or precipitated out of lysate using antibodies in IP. Affinity chromatography can also be used which is a method of separating biochemical mixtures based on highly specific biological interaction. Specific antigen can be covalently coupled to a solid support and allow supernatant with the testing antibody to flow through so that only specific antibodies will be bound to the antigens. The assay used in this study can only detect antigens in a non-quantitative way with chromatic display of antigen localisation. Instead radioimmunoluminography (RILG) can be used for quantitative measurements of antigen concentration along with its distribution in histological sections.8 Radiolabelled antibodies against specific antigen can be applied to tissue sections and bound antibodies are mapped by phosphor imaging. Radioactivity detected in each pixel of the digital image will be proportional to antigen concentration if saturating antibody concentration is used. Future Perspective of Antibody Targeted Cancer Therapy The successful application of scFv MFE-23 with a Myc-tag at its C-terminus for detection in immunohistochemistry proves that the attachment of small molecules to the antibody will not affect its specificity for CEA. Thus the scFv chain can be conjugated to cytotoxic reagents or to be used for ADEPT as mentioned previously. The potential clinical efficacy of scFv MFE-23:enzyme fusion protein has been shown in nude mice with human colon adenocarcinoma xenografts by the Bhatia group.9 Moreover, scFv MFE-23 can be used in radioimmunoguided surgery (RIGS) based on the pre-operative injection of a radiolabelled anti-tumor antibody to detect tumour deposits during surgery. A Phase I clinical trial of RIGS using 125iodine-labelled MFE- 23-his scFv has reported good selective localisation at sites of primary colorectal cancer and metastases.10 As illustrated by MFE-23 scFv fragment, antibody targeting has the potential for selective imaging or delivery of anti-cancer molecules. Antibodies can be engineered to modify their biological properties with increased specificity and functionality. This is achieved by reducing antibody size, altering valency, and fusing to different molecules to improve therapeutic efficiency. Scientists have been trying to produce smaller antibody fragments but retaining specific binding to antigens, in order to minimize immunogenicity and achieve better tumour penetration. Continuous research on the specificity and stability of these fragments, and hunting for more tumour-specific antigens are required to further expand the field of antibody-targeted cancer therapies. Slide 3 is a negative control for slide 1 which was treated with the 4 stage anti-Myc technique. Slide 4 is a negative control for slide 2 which was treated with the 3 stage mouse monoclonal technique. Slide 5 is a negative control for slide 2 as well but without biotin/avidin blocking. Slide 1 was treated with the 4 stage anti-Myc technique, and slide 2 was treated with the 3 stage mouse monoclonal technique. REFERENCES Hanahan D, Weinberg RA. The hallmarks of cancer. Cell. 2000 Jan 7;100(1):57-70. Baron, E. J. 1996. Classification. In S. Baron et al., eds. Barons Medical Microbiology, 4th edition. University of Texas Medical Branch. Gura, T. Therapeutic antibodies:Magic bullets hit the target. Nature 417, 584-586 (6 June 2002) Carter, P. Improving the efficacy of antibody-based cancer therapies Nature Reviews Cancer 1, 118-129 (November 2001) Sten Hammarstrà ¶m. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Seminars in Cancer Biology. Volume 9, Issue 2, April 1999, Pages 67-81 A. Mayer, K. A. Chester, et al. Taking engineered anti-CEA antibodies to the clinic. Journal of Immunological Methods. Volume 231, Issues 1-2, 10 December 1999, Pages 261-273 Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577-80. GBoxer, SStuart-Smith, et al. Radioimmunoluminography: a tool for relating tissue antigen concentration to clinical outcome. British Journal of Cancer (1999) 80, 922-926. Bhatia J, Sharma SK, et al. Catalytic activity of an in vivo tumor targeted anti-CEA scFv:carboxypeptidase G2 fusion protein. Int J Cancer. 2000 Feb 15;85(4):571-7. Mayer, A., et al. Radioimmunoguided Surgery in Colorectal Cancer Using a Genetically Engineered Anti-CEA Single-Chain Fv Antibody. Clinical Cancer Research May 2000 6; 1711. APPENDIX Appendix 1 Experimental Procedures (UCL Cancer MSc Lab Practical 2 Handout, 12-13Nov09) E. Coli growth curves E.coli culture transformed with pUC119 containing either His- or Myc- tagged scFv MFE-23 (Culture 1 2) were incubated overnight. The expression of pUC119 was controlled by the lac operon, which could be induced by either lactose or lactose analogue isopropyl-1-ß-D-thiogalactoside (IPTG). The vector also encoded for ampicillin resistance. 30 µl of the media was added to 15ml growth media with ampicillin selection and 0.05% glucose. Both tubes on loose caps were placed into a 37? shaker at 225rpm and time was taken as t=0. Optical density (OD) of the culture was measured with a spectrophotometer at 600nm from t=0 at a 30min interval until reading reached OD=0.9. 1mM IPTG was added to each culture and the tubes were incubated at 30? overnight with shaking. A negative control was set up using the same volume of growth media 2xYT with 1mM IPTG for overnight incubation. ELISA assay 100 µl of 10 µg/ml CEA antigen was applied to strips 1-6, A-H of the 96-well ELISA plate. 100 µl of PBS was applied to strips 7-12, A-H as negative controls. The plate was covered with plastic film and incubated for 1 hour at room temperature. Each well was rinsed 4 times with PBS and blocked with 200 µl 5% Marvel milk/PBS. The plate was covered and incubated overnight at 4?. The plate was washed with PBS 4 times after the overnight incubation. Overnight bacterial cultures and the negative control were centrifuged at 4000rpm for 20minutes to obtain the supernatant. 100 µl of each supernatant sample and negative control was added to the ELISA plate according to the format diagram as seen in Appendix 2. The covered plate was left at room temperature for an hour. The plate was then rinsed twice with 0.1% Tween-20/PBS and 4 times with PBS. 100 µl of secondary antibodies rabbit anti-MFE23 polysera, mouse monoclonal anti-HIS tag (TetraHis, Qiagen) and mouse monoclonal anti-MYC (Sigma) with a concentration of 1:1000 in 1% blocking solution was added to the wells according to the format diagram: anti-His A1-12 and B1-12; anti-Myc C1-12 and D1-12; anti-MFE E1-12 and F1-12. The covered plate was left at room temperature for an hour. The plate was washed again twice with 0.1% Tween-20/PBS and 4 times with PBS. 100 µl horseradish peroxidase (HRP) conjugated antibodies against the secondary antibodies 1:1000 goat anti-rabbit HRP (sigma) and 1:500 sheep anti-mouse HRP (Sigma) in 1% blocking solution was added to the corresponding wells. The covered plate was left at room temperature for an hour, and then rinsed twice with 0.1% Tween-20/PBS and 4 times with PBS. 100 µl of OPD substrate buffer was added to each well to detect the presence of HRP, giving a yellow-orange product in the case of positive outcome. 100 µl of 4M HCl was added when the colour has developed, and OD was measured at 490nm with an automated plate reader. Immunohistochemistry assay Five glass slides, each containing 2 colonic adenocarcinoma, 1 normal colon and 1 normal liver tissue sections were removed from the freezer and air dried for 5minutes. Slides were fixed in acetone in 10minutes and rinsed in tap water for 2minutes. Slides were flooded with PBS and Avidin blocking solution was applied to slides 1-4 for 10 minutes. Slides 1-4 were rinsed in PBS and applied with Biotin blocking solution for 10minutes followed by PBS wash. PBS was removed from slide 1 and flooded with 1:20 normal horse serum in PBS for 15minutes. Slide 1 was drained and applied with bacterial supernatant containing Myc-tagged scFv MFE-23 antibodies for 45minutes. Slide 1 was then rinsed with PBS thrice over 10minutes, and 1:20 normal horse serum in PBS was added to slides 2-5 for 15minutes. 20 µg/ml of mouse monoclonal anti-Myc antibody (Qiagen) in PBS was added to slides 13 and left for 35minutes. 20 µg/ml of A5B7 anti-CEA antibody was added to slide 2 for 35minutes. Slides 4-5 were washed in PBS. Slides 1-3 were rinsed with PBS thrice over 10minutes. 1:200 biotinylated horse anti-mouse immunoglobulins in PBS with 5% normal human serum was added to all slides and incubated for 35minutes. All slides were rinsed with PBS thrice over 10minutes. The avidin biotin-peroxidase complexes reagent (ABC reagent) was added to all slides, followed by PBS wash 3 times over 10minutes. 0.03% of hydrogen peroxide in 1mg/ml 3,3,diaminobenzidene tetrahydrochloride solution was applied to all slides immediately and left for 5minutes. Slides were washed with tap water. All slides were stained in Harris haematoxylin for 40seconds, rinsed in tap water and left for 5minutes for colour to develop. Slides were then dehydrated through graded alcohols (70/95/100%), cleared in inhibisol and coverslip using DPX mountant. The slides were then ready for observations under the light microscope.